|Cy5 NHS ester (non-sulfonated)
Labeling of amino-groups in biomolecules.
Sample solution is provided at 25 µL, 10mM.
|Cas No.||N/A||SDF||Download SDF|
|Chemical Name||(2,5-dioxopyrrolidin-1-yl) 6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoate|
|Solubility||≥30.8mg/mL in DMSO||Storage||24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.|
|Physical Appearance||A solid||Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Excitation max (nm)||646||Emission max (nm)||662|
|Extinction Coefficient (M-1cm-1)||250000||Quantum Yield||0.2|
Non-sulfonated cyanines are available for the labeling of many targets including:
|• Soluble proteins, which are tolerant to addition of organic co-solvent||• Fluorescence polarization (FP)|
|• Antibodies (with 5-10% of DMSO/DMF)||• Fluorescence resonance energy transfer (FRET)|
|• DNA and oligonucleotides||• Time-resolved fluorescence energy transfer (TR-FRET)|
|• Peptides||• Fluorescence intensity (FI)|
|• Many small molecules|
|• Reactions in organic media (dichloromethane, acetonitrile)|
Cyanine5 NHS ester is a ideal reactive dye for the labeling of peptides, proteins, and oligonucleotides which contain the amino-groups. Recently, among the life science research and diagnostics, Dye color quantity can be detected in gel electrophoresis by naked eye as the dye color is very intense and as small as 1 nanomol. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. For more sophisticated targets such as easily degradable proteins, when use of DMF or DMSO is undesirable, consider using water-soluble Cy5 NHS ester which does not require co-solvent, and has very similar fluorescent properties.
In mice, Cy5-labeled polyplexes was intravenous injected to evaluate the targeting ability of polyplexes in tumor-bearing nude mice. Heart, liver, spleen, lung, kidney and tumor, were removed after the mice were sacrificed and the fluorescence intensities of Cy5-labeled polyplexes were immediately examined .
 Chen, K. ; Chen, Q.; Wang, K.; Zhu, J.; Li, W.; Li, W.; Qiu, L.; Guan, G.; Qiao, M.; Zhao, X.; Hu, H.; Chen, D. Synthesis and characterization of a PAMAM-OH derivative containing an acid-labile β-thiopropionate bond for gene delivery. International Journal of Pharmaceutics, 2016, 509(1–2), 314–327.